SignalSilence® Pool p42 MAPK siRNA
| Name | SignalSilence® Pool p42 MAPK siRNA |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 6391 |
| Category | siRNA |
| Applications | Gene Silencing |
| Species Reactivities | Human, Monkey |
| Gene | MAPK1 |
| Description | SignalSilence® Pool p42 MAP Kinase siRNA from Cell Signaling Technology allows the researcher to specifically inhibit p42 MAP Kinase expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from HeLa cells transfected with 20 nM control siRNA #6201 (-) or 2, 5, or 20 nM pool 42 MAPK siRNA, using p44/42 MAP Kinase Antibody #9102 (upper), p38 MAP Kinase Antibody #9212 (middle) and SAPK/JNK (56G8) Rabbit mAb #9258 (lower) in combination with ATP-Citrate Lyase Antibody #4332. The 44/42 MAP Kinase Antibody confirms silencing of p42 MAP Kinase expression, and ATP-Citrate Lyase Antibody is used to control for loading and specificity of pool p42 MAP Kinase siRNA. The experiment also demonstrates specifically that the pool p42 MAPK siRNA does not interfere with the expression of p44 MAPK, p38 MAPK or SAPK/JNKs.
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.